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    Bio-Rad powerpac hc high current power supply bio rad
    Powerpac Hc High Current Power Supply Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 301 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/powerpac+hc+high+current+power+supply+bio+rad/PowerPac+HC+Power+Supply/pm37059091-215-222-227
    Average 96 stars, based on 301 article reviews
    powerpac hc high current power supply bio rad - by Bioz Stars, 2026-09
    96/100 stars

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    Article Title: Compartmentalization of the SUMO/RNF4 pathway by SLX4 drives DNA repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Imidazole Sigma-Aldrich Cat# I5513; CAS: 288-32-4 Formaldehyde 32% Solution Euromedex Cat# EM-15714 Sodium phosphate monobasic Sigma-Aldrich Cat# S0751; CAS: 7558-80-7 Sodium phosphate dibasic Sigma-Aldrich Cat# S0876; CAS: 7558-79-4 TWEEN 20 Sigma-Aldrich Cat# P9416; CAS: 9005-64-5 5-Ethynyl-20-deoxyuridine Santa Cruz Biotechnology Cat# sc-284628; CAS: 61135-33-9 (S)-(+)-Camptothecin Sigma-Aldrich Cat# C9911; CAS: 7689-03-4 Hydroxyurea Sigma-Aldrich Cat# H8627; CAS: 127-07-1 Mitomycine C Roche Cat# 10107409001; CAS: 50-07-7 Urea Sigma-Aldrich Cat# U5378; CAS: 57-13-6 SPY650-DNA Spirochrome Cat# SC501 SPY595-DNA Spirochrome Cat# SC301 MEM NEAA (100X) Gibco Cat# 11140050 HaloPROTAC3 Promega Cat# GA3110 RNAse A solution Merck Cat# 70856 Trypsin Gold, Mass Spectrometry Grade Promega Cat# V5280 Acetonitrile, Optima LC/MS Grade Fisher Chemical Cat# A955-1 Formic Acid, 99.0+%, Optima LC/MS Grade Fisher Chemical Cat# A117-50 Recombinant Human SUMO E1 (SAE1/UBA2) Protein Boston Biochem Cat# E-315 Recombinant Human UBE2I/Ubc9 Protein Boston Biochem Cat# E2-645-100 Recombinant Human SUMO2 Protein Boston Biochem Cat# UL-752 Critical Commercial Assays Clarity western ECL substrate Bio-Rad Cat# 170-5061 Clarity max western ECL substrate Bio-Rad Cat# 1705062 Criterion TGX stain free gel 7,5% Bio-Rad Cat# 5678024 Criterion TGX stain free gel 4-15% Bio-Rad Cat# 5678084 Criterion TGX stain free gel 10% Bio-Rad Cat# 5671034 Mini-PROTEAN TGX Stain Free Gels, 7.5% Bio-Rad Cat# 4568023 Mini-PROTEAN TGX Stain Free Gels, 4-15% Bio-Rad Cat# 4568083 Mini-PROTEAN TGX Stain Free Gels, 10% Bio-Rad Cat# 4568033 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Midi, 10 pack Bio-Rad Cat# 1704159 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Mini, 10 pack Bio-Rad Cat# 1704158 Color Prestained Protein Standard, Broad Range BioLAbs Cat# P7712S Lipofectamine 2000 ThermoFisher Scientific Cat# 11668-019 Quick Start Bradford 1x Dye Reagent Bio-Rad Cat# 500-0205 Amersham Hyperfilm ECL (8 3 10") Dominique Dutscher Cat# 28906839 Quick change multi site directed Agilent technologies Cat# 200515–5 Infusion Kit HD cloning Takara Bio Cat# 639649 Prolong gold antifade ThermoFisher Scientific Cat# P10144 TALON Metal Affinity Resin Takara Cat# 635501 INTERFERin Polyplus Cat# 409-01 Amersham Protran 0.45 NC nitrocellulose Western blotting membranes Cytiva Cat# 10600002 GFP Trap Agarose Kit Chromotek Cat# ABIN509397; RRID:AB_2884031 (Continued on next page) Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023

    Recombinant:

    Article Title: Compartmentalization of the SUMO/RNF4 pathway by SLX4 drives DNA repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Imidazole Sigma-Aldrich Cat# I5513; CAS: 288-32-4 Formaldehyde 32% Solution Euromedex Cat# EM-15714 Sodium phosphate monobasic Sigma-Aldrich Cat# S0751; CAS: 7558-80-7 Sodium phosphate dibasic Sigma-Aldrich Cat# S0876; CAS: 7558-79-4 TWEEN 20 Sigma-Aldrich Cat# P9416; CAS: 9005-64-5 5-Ethynyl-20-deoxyuridine Santa Cruz Biotechnology Cat# sc-284628; CAS: 61135-33-9 (S)-(+)-Camptothecin Sigma-Aldrich Cat# C9911; CAS: 7689-03-4 Hydroxyurea Sigma-Aldrich Cat# H8627; CAS: 127-07-1 Mitomycine C Roche Cat# 10107409001; CAS: 50-07-7 Urea Sigma-Aldrich Cat# U5378; CAS: 57-13-6 SPY650-DNA Spirochrome Cat# SC501 SPY595-DNA Spirochrome Cat# SC301 MEM NEAA (100X) Gibco Cat# 11140050 HaloPROTAC3 Promega Cat# GA3110 RNAse A solution Merck Cat# 70856 Trypsin Gold, Mass Spectrometry Grade Promega Cat# V5280 Acetonitrile, Optima LC/MS Grade Fisher Chemical Cat# A955-1 Formic Acid, 99.0+%, Optima LC/MS Grade Fisher Chemical Cat# A117-50 Recombinant Human SUMO E1 (SAE1/UBA2) Protein Boston Biochem Cat# E-315 Recombinant Human UBE2I/Ubc9 Protein Boston Biochem Cat# E2-645-100 Recombinant Human SUMO2 Protein Boston Biochem Cat# UL-752 Critical Commercial Assays Clarity western ECL substrate Bio-Rad Cat# 170-5061 Clarity max western ECL substrate Bio-Rad Cat# 1705062 Criterion TGX stain free gel 7,5% Bio-Rad Cat# 5678024 Criterion TGX stain free gel 4-15% Bio-Rad Cat# 5678084 Criterion TGX stain free gel 10% Bio-Rad Cat# 5671034 Mini-PROTEAN TGX Stain Free Gels, 7.5% Bio-Rad Cat# 4568023 Mini-PROTEAN TGX Stain Free Gels, 4-15% Bio-Rad Cat# 4568083 Mini-PROTEAN TGX Stain Free Gels, 10% Bio-Rad Cat# 4568033 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Midi, 10 pack Bio-Rad Cat# 1704159 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Mini, 10 pack Bio-Rad Cat# 1704158 Color Prestained Protein Standard, Broad Range BioLAbs Cat# P7712S Lipofectamine 2000 ThermoFisher Scientific Cat# 11668-019 Quick Start Bradford 1x Dye Reagent Bio-Rad Cat# 500-0205 Amersham Hyperfilm ECL (8 3 10") Dominique Dutscher Cat# 28906839 Quick change multi site directed Agilent technologies Cat# 200515–5 Infusion Kit HD cloning Takara Bio Cat# 639649 Prolong gold antifade ThermoFisher Scientific Cat# P10144 TALON Metal Affinity Resin Takara Cat# 635501 INTERFERin Polyplus Cat# 409-01 Amersham Protran 0.45 NC nitrocellulose Western blotting membranes Cytiva Cat# 10600002 GFP Trap Agarose Kit Chromotek Cat# ABIN509397; RRID:AB_2884031 (Continued on next page) Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023

    Expressing:

    Article Title: Compartmentalization of the SUMO/RNF4 pathway by SLX4 drives DNA repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Imidazole Sigma-Aldrich Cat# I5513; CAS: 288-32-4 Formaldehyde 32% Solution Euromedex Cat# EM-15714 Sodium phosphate monobasic Sigma-Aldrich Cat# S0751; CAS: 7558-80-7 Sodium phosphate dibasic Sigma-Aldrich Cat# S0876; CAS: 7558-79-4 TWEEN 20 Sigma-Aldrich Cat# P9416; CAS: 9005-64-5 5-Ethynyl-20-deoxyuridine Santa Cruz Biotechnology Cat# sc-284628; CAS: 61135-33-9 (S)-(+)-Camptothecin Sigma-Aldrich Cat# C9911; CAS: 7689-03-4 Hydroxyurea Sigma-Aldrich Cat# H8627; CAS: 127-07-1 Mitomycine C Roche Cat# 10107409001; CAS: 50-07-7 Urea Sigma-Aldrich Cat# U5378; CAS: 57-13-6 SPY650-DNA Spirochrome Cat# SC501 SPY595-DNA Spirochrome Cat# SC301 MEM NEAA (100X) Gibco Cat# 11140050 HaloPROTAC3 Promega Cat# GA3110 RNAse A solution Merck Cat# 70856 Trypsin Gold, Mass Spectrometry Grade Promega Cat# V5280 Acetonitrile, Optima LC/MS Grade Fisher Chemical Cat# A955-1 Formic Acid, 99.0+%, Optima LC/MS Grade Fisher Chemical Cat# A117-50 Recombinant Human SUMO E1 (SAE1/UBA2) Protein Boston Biochem Cat# E-315 Recombinant Human UBE2I/Ubc9 Protein Boston Biochem Cat# E2-645-100 Recombinant Human SUMO2 Protein Boston Biochem Cat# UL-752 Critical Commercial Assays Clarity western ECL substrate Bio-Rad Cat# 170-5061 Clarity max western ECL substrate Bio-Rad Cat# 1705062 Criterion TGX stain free gel 7,5% Bio-Rad Cat# 5678024 Criterion TGX stain free gel 4-15% Bio-Rad Cat# 5678084 Criterion TGX stain free gel 10% Bio-Rad Cat# 5671034 Mini-PROTEAN TGX Stain Free Gels, 7.5% Bio-Rad Cat# 4568023 Mini-PROTEAN TGX Stain Free Gels, 4-15% Bio-Rad Cat# 4568083 Mini-PROTEAN TGX Stain Free Gels, 10% Bio-Rad Cat# 4568033 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Midi, 10 pack Bio-Rad Cat# 1704159 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Mini, 10 pack Bio-Rad Cat# 1704158 Color Prestained Protein Standard, Broad Range BioLAbs Cat# P7712S Lipofectamine 2000 ThermoFisher Scientific Cat# 11668-019 Quick Start Bradford 1x Dye Reagent Bio-Rad Cat# 500-0205 Amersham Hyperfilm ECL (8 3 10") Dominique Dutscher Cat# 28906839 Quick change multi site directed Agilent technologies Cat# 200515–5 Infusion Kit HD cloning Takara Bio Cat# 639649 Prolong gold antifade ThermoFisher Scientific Cat# P10144 TALON Metal Affinity Resin Takara Cat# 635501 INTERFERin Polyplus Cat# 409-01 Amersham Protran 0.45 NC nitrocellulose Western blotting membranes Cytiva Cat# 10600002 GFP Trap Agarose Kit Chromotek Cat# ABIN509397; RRID:AB_2884031 (Continued on next page) Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023

    Software:

    Article Title: Compartmentalization of the SUMO/RNF4 pathway by SLX4 drives DNA repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Imidazole Sigma-Aldrich Cat# I5513; CAS: 288-32-4 Formaldehyde 32% Solution Euromedex Cat# EM-15714 Sodium phosphate monobasic Sigma-Aldrich Cat# S0751; CAS: 7558-80-7 Sodium phosphate dibasic Sigma-Aldrich Cat# S0876; CAS: 7558-79-4 TWEEN 20 Sigma-Aldrich Cat# P9416; CAS: 9005-64-5 5-Ethynyl-20-deoxyuridine Santa Cruz Biotechnology Cat# sc-284628; CAS: 61135-33-9 (S)-(+)-Camptothecin Sigma-Aldrich Cat# C9911; CAS: 7689-03-4 Hydroxyurea Sigma-Aldrich Cat# H8627; CAS: 127-07-1 Mitomycine C Roche Cat# 10107409001; CAS: 50-07-7 Urea Sigma-Aldrich Cat# U5378; CAS: 57-13-6 SPY650-DNA Spirochrome Cat# SC501 SPY595-DNA Spirochrome Cat# SC301 MEM NEAA (100X) Gibco Cat# 11140050 HaloPROTAC3 Promega Cat# GA3110 RNAse A solution Merck Cat# 70856 Trypsin Gold, Mass Spectrometry Grade Promega Cat# V5280 Acetonitrile, Optima LC/MS Grade Fisher Chemical Cat# A955-1 Formic Acid, 99.0+%, Optima LC/MS Grade Fisher Chemical Cat# A117-50 Recombinant Human SUMO E1 (SAE1/UBA2) Protein Boston Biochem Cat# E-315 Recombinant Human UBE2I/Ubc9 Protein Boston Biochem Cat# E2-645-100 Recombinant Human SUMO2 Protein Boston Biochem Cat# UL-752 Critical Commercial Assays Clarity western ECL substrate Bio-Rad Cat# 170-5061 Clarity max western ECL substrate Bio-Rad Cat# 1705062 Criterion TGX stain free gel 7,5% Bio-Rad Cat# 5678024 Criterion TGX stain free gel 4-15% Bio-Rad Cat# 5678084 Criterion TGX stain free gel 10% Bio-Rad Cat# 5671034 Mini-PROTEAN TGX Stain Free Gels, 7.5% Bio-Rad Cat# 4568023 Mini-PROTEAN TGX Stain Free Gels, 4-15% Bio-Rad Cat# 4568083 Mini-PROTEAN TGX Stain Free Gels, 10% Bio-Rad Cat# 4568033 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Midi, 10 pack Bio-Rad Cat# 1704159 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Mini, 10 pack Bio-Rad Cat# 1704158 Color Prestained Protein Standard, Broad Range BioLAbs Cat# P7712S Lipofectamine 2000 ThermoFisher Scientific Cat# 11668-019 Quick Start Bradford 1x Dye Reagent Bio-Rad Cat# 500-0205 Amersham Hyperfilm ECL (8 3 10") Dominique Dutscher Cat# 28906839 Quick change multi site directed Agilent technologies Cat# 200515–5 Infusion Kit HD cloning Takara Bio Cat# 639649 Prolong gold antifade ThermoFisher Scientific Cat# P10144 TALON Metal Affinity Resin Takara Cat# 635501 INTERFERin Polyplus Cat# 409-01 Amersham Protran 0.45 NC nitrocellulose Western blotting membranes Cytiva Cat# 10600002 GFP Trap Agarose Kit Chromotek Cat# ABIN509397; RRID:AB_2884031 (Continued on next page) Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023

    Electrophoresis:

    Article Title: Compartmentalization of the SUMO/RNF4 pathway by SLX4 drives DNA repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Imidazole Sigma-Aldrich Cat# I5513; CAS: 288-32-4 Formaldehyde 32% Solution Euromedex Cat# EM-15714 Sodium phosphate monobasic Sigma-Aldrich Cat# S0751; CAS: 7558-80-7 Sodium phosphate dibasic Sigma-Aldrich Cat# S0876; CAS: 7558-79-4 TWEEN 20 Sigma-Aldrich Cat# P9416; CAS: 9005-64-5 5-Ethynyl-20-deoxyuridine Santa Cruz Biotechnology Cat# sc-284628; CAS: 61135-33-9 (S)-(+)-Camptothecin Sigma-Aldrich Cat# C9911; CAS: 7689-03-4 Hydroxyurea Sigma-Aldrich Cat# H8627; CAS: 127-07-1 Mitomycine C Roche Cat# 10107409001; CAS: 50-07-7 Urea Sigma-Aldrich Cat# U5378; CAS: 57-13-6 SPY650-DNA Spirochrome Cat# SC501 SPY595-DNA Spirochrome Cat# SC301 MEM NEAA (100X) Gibco Cat# 11140050 HaloPROTAC3 Promega Cat# GA3110 RNAse A solution Merck Cat# 70856 Trypsin Gold, Mass Spectrometry Grade Promega Cat# V5280 Acetonitrile, Optima LC/MS Grade Fisher Chemical Cat# A955-1 Formic Acid, 99.0+%, Optima LC/MS Grade Fisher Chemical Cat# A117-50 Recombinant Human SUMO E1 (SAE1/UBA2) Protein Boston Biochem Cat# E-315 Recombinant Human UBE2I/Ubc9 Protein Boston Biochem Cat# E2-645-100 Recombinant Human SUMO2 Protein Boston Biochem Cat# UL-752 Critical Commercial Assays Clarity western ECL substrate Bio-Rad Cat# 170-5061 Clarity max western ECL substrate Bio-Rad Cat# 1705062 Criterion TGX stain free gel 7,5% Bio-Rad Cat# 5678024 Criterion TGX stain free gel 4-15% Bio-Rad Cat# 5678084 Criterion TGX stain free gel 10% Bio-Rad Cat# 5671034 Mini-PROTEAN TGX Stain Free Gels, 7.5% Bio-Rad Cat# 4568023 Mini-PROTEAN TGX Stain Free Gels, 4-15% Bio-Rad Cat# 4568083 Mini-PROTEAN TGX Stain Free Gels, 10% Bio-Rad Cat# 4568033 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Midi, 10 pack Bio-Rad Cat# 1704159 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Mini, 10 pack Bio-Rad Cat# 1704158 Color Prestained Protein Standard, Broad Range BioLAbs Cat# P7712S Lipofectamine 2000 ThermoFisher Scientific Cat# 11668-019 Quick Start Bradford 1x Dye Reagent Bio-Rad Cat# 500-0205 Amersham Hyperfilm ECL (8 3 10") Dominique Dutscher Cat# 28906839 Quick change multi site directed Agilent technologies Cat# 200515–5 Infusion Kit HD cloning Takara Bio Cat# 639649 Prolong gold antifade ThermoFisher Scientific Cat# P10144 TALON Metal Affinity Resin Takara Cat# 635501 INTERFERin Polyplus Cat# 409-01 Amersham Protran 0.45 NC nitrocellulose Western blotting membranes Cytiva Cat# 10600002 GFP Trap Agarose Kit Chromotek Cat# ABIN509397; RRID:AB_2884031 (Continued on next page) Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023

    Article Title: Design of Artificial Alcohol Oxidases: Alcohol Dehydrogenase–NADPH Oxidase Fusions for Continuous Oxidations
    Article Snippet: .. The samples were loaded onto a precast SDS‐PAGE gel (GenScript, USA), and the gels were run in a Mini‐PROTEAN Tetra Vertical Electrophoresis Cell (Bio‐Rad) with the current being applied by using a PowerPac HC High‐Current Power Supply (Bio‐Rad) set at 120 V. When the blue front of the loading dye reached the bottom of the gel, after about 70 min, the gel was removed from the chamber, and stained with Coomassie InstantBlue (Expedeon, US). .. An UV/Vis absorption spectrum from 200 to 700 nm (V‐330 Spectrophotometer, JASCO) was taken of each purified fusion protein diluted 1:10 in buffer (50 m m Tris ⋅ HCl, pH 8) in a quartz cuvette (Hellma Analytics, Germany).

    Dot Blot:

    Article Title: Compartmentalization of the SUMO/RNF4 pathway by SLX4 drives DNA repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Imidazole Sigma-Aldrich Cat# I5513; CAS: 288-32-4 Formaldehyde 32% Solution Euromedex Cat# EM-15714 Sodium phosphate monobasic Sigma-Aldrich Cat# S0751; CAS: 7558-80-7 Sodium phosphate dibasic Sigma-Aldrich Cat# S0876; CAS: 7558-79-4 TWEEN 20 Sigma-Aldrich Cat# P9416; CAS: 9005-64-5 5-Ethynyl-20-deoxyuridine Santa Cruz Biotechnology Cat# sc-284628; CAS: 61135-33-9 (S)-(+)-Camptothecin Sigma-Aldrich Cat# C9911; CAS: 7689-03-4 Hydroxyurea Sigma-Aldrich Cat# H8627; CAS: 127-07-1 Mitomycine C Roche Cat# 10107409001; CAS: 50-07-7 Urea Sigma-Aldrich Cat# U5378; CAS: 57-13-6 SPY650-DNA Spirochrome Cat# SC501 SPY595-DNA Spirochrome Cat# SC301 MEM NEAA (100X) Gibco Cat# 11140050 HaloPROTAC3 Promega Cat# GA3110 RNAse A solution Merck Cat# 70856 Trypsin Gold, Mass Spectrometry Grade Promega Cat# V5280 Acetonitrile, Optima LC/MS Grade Fisher Chemical Cat# A955-1 Formic Acid, 99.0+%, Optima LC/MS Grade Fisher Chemical Cat# A117-50 Recombinant Human SUMO E1 (SAE1/UBA2) Protein Boston Biochem Cat# E-315 Recombinant Human UBE2I/Ubc9 Protein Boston Biochem Cat# E2-645-100 Recombinant Human SUMO2 Protein Boston Biochem Cat# UL-752 Critical Commercial Assays Clarity western ECL substrate Bio-Rad Cat# 170-5061 Clarity max western ECL substrate Bio-Rad Cat# 1705062 Criterion TGX stain free gel 7,5% Bio-Rad Cat# 5678024 Criterion TGX stain free gel 4-15% Bio-Rad Cat# 5678084 Criterion TGX stain free gel 10% Bio-Rad Cat# 5671034 Mini-PROTEAN TGX Stain Free Gels, 7.5% Bio-Rad Cat# 4568023 Mini-PROTEAN TGX Stain Free Gels, 4-15% Bio-Rad Cat# 4568083 Mini-PROTEAN TGX Stain Free Gels, 10% Bio-Rad Cat# 4568033 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Midi, 10 pack Bio-Rad Cat# 1704159 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Mini, 10 pack Bio-Rad Cat# 1704158 Color Prestained Protein Standard, Broad Range BioLAbs Cat# P7712S Lipofectamine 2000 ThermoFisher Scientific Cat# 11668-019 Quick Start Bradford 1x Dye Reagent Bio-Rad Cat# 500-0205 Amersham Hyperfilm ECL (8 3 10") Dominique Dutscher Cat# 28906839 Quick change multi site directed Agilent technologies Cat# 200515–5 Infusion Kit HD cloning Takara Bio Cat# 639649 Prolong gold antifade ThermoFisher Scientific Cat# P10144 TALON Metal Affinity Resin Takara Cat# 635501 INTERFERin Polyplus Cat# 409-01 Amersham Protran 0.45 NC nitrocellulose Western blotting membranes Cytiva Cat# 10600002 GFP Trap Agarose Kit Chromotek Cat# ABIN509397; RRID:AB_2884031 (Continued on next page) Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023

    High Performance Liquid Chromatography:

    Article Title: Compartmentalization of the SUMO/RNF4 pathway by SLX4 drives DNA repair.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Imidazole Sigma-Aldrich Cat# I5513; CAS: 288-32-4 Formaldehyde 32% Solution Euromedex Cat# EM-15714 Sodium phosphate monobasic Sigma-Aldrich Cat# S0751; CAS: 7558-80-7 Sodium phosphate dibasic Sigma-Aldrich Cat# S0876; CAS: 7558-79-4 TWEEN 20 Sigma-Aldrich Cat# P9416; CAS: 9005-64-5 5-Ethynyl-20-deoxyuridine Santa Cruz Biotechnology Cat# sc-284628; CAS: 61135-33-9 (S)-(+)-Camptothecin Sigma-Aldrich Cat# C9911; CAS: 7689-03-4 Hydroxyurea Sigma-Aldrich Cat# H8627; CAS: 127-07-1 Mitomycine C Roche Cat# 10107409001; CAS: 50-07-7 Urea Sigma-Aldrich Cat# U5378; CAS: 57-13-6 SPY650-DNA Spirochrome Cat# SC501 SPY595-DNA Spirochrome Cat# SC301 MEM NEAA (100X) Gibco Cat# 11140050 HaloPROTAC3 Promega Cat# GA3110 RNAse A solution Merck Cat# 70856 Trypsin Gold, Mass Spectrometry Grade Promega Cat# V5280 Acetonitrile, Optima LC/MS Grade Fisher Chemical Cat# A955-1 Formic Acid, 99.0+%, Optima LC/MS Grade Fisher Chemical Cat# A117-50 Recombinant Human SUMO E1 (SAE1/UBA2) Protein Boston Biochem Cat# E-315 Recombinant Human UBE2I/Ubc9 Protein Boston Biochem Cat# E2-645-100 Recombinant Human SUMO2 Protein Boston Biochem Cat# UL-752 Critical Commercial Assays Clarity western ECL substrate Bio-Rad Cat# 170-5061 Clarity max western ECL substrate Bio-Rad Cat# 1705062 Criterion TGX stain free gel 7,5% Bio-Rad Cat# 5678024 Criterion TGX stain free gel 4-15% Bio-Rad Cat# 5678084 Criterion TGX stain free gel 10% Bio-Rad Cat# 5671034 Mini-PROTEAN TGX Stain Free Gels, 7.5% Bio-Rad Cat# 4568023 Mini-PROTEAN TGX Stain Free Gels, 4-15% Bio-Rad Cat# 4568083 Mini-PROTEAN TGX Stain Free Gels, 10% Bio-Rad Cat# 4568033 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Midi, 10 pack Bio-Rad Cat# 1704159 Trans-Blot Turbo Transfer Pack 0,2mm Nitrocellulose Mini, 10 pack Bio-Rad Cat# 1704158 Color Prestained Protein Standard, Broad Range BioLAbs Cat# P7712S Lipofectamine 2000 ThermoFisher Scientific Cat# 11668-019 Quick Start Bradford 1x Dye Reagent Bio-Rad Cat# 500-0205 Amersham Hyperfilm ECL (8 3 10") Dominique Dutscher Cat# 28906839 Quick change multi site directed Agilent technologies Cat# 200515–5 Infusion Kit HD cloning Takara Bio Cat# 639649 Prolong gold antifade ThermoFisher Scientific Cat# P10144 TALON Metal Affinity Resin Takara Cat# 635501 INTERFERin Polyplus Cat# 409-01 Amersham Protran 0.45 NC nitrocellulose Western blotting membranes Cytiva Cat# 10600002 GFP Trap Agarose Kit Chromotek Cat# ABIN509397; RRID:AB_2884031 (Continued on next page) Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 e3 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Deposited Data Raw data This paper Mendeley Dataset: https://doi.org/10.17632/c5h63ykhpn.1 Experimental Models: Cell Lines Flp-In T-REx 293 Invitrogen Cat# R78007; RRID:CVCL_U427 SLX4-null (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A SLX4-complemented (RA3331/E6E7/hTERT) Gift from Dr. Agata Smogorzewska N/A HEK293T Sigma-Aldrich Cat# 12022001; RRID:CVCL_0063 CL-HJL-0104-A KI HEK 293T Biocytogen N/A Oligonucleotides siGENOME Non-Targeting siRNA Control Pools Dharmacon_Horizon Discovery Cat# ID:D-001206-13-20 siGENOME Human SLX4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-014895-01-0020 siGENOME Human RNF4 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006557-03-0020 siGENOME Human UBE2I siRNA Dharmacon_Horizon Discovery Cat# ID:M-004910-00-0020 siGENOME Human SENP6 siRNA Dharmacon_Horizon Discovery Cat# ID:M-006044-01-0020 The oligonucleotides used in this study are listed in Table S2 N/A Recombinant DNA pCDNA5_FRT_TO_TurboID-mCherry-Cry2 Addgene Cat# 166504; http://n2t.net/addgene:166504; RRID:Addgene_166504 pOG44 Flp-Recombinase Expression Vector ThermoFisher Scientific Cat# V600520 pLenti-CMV-Blast-PIP-FUCCI Addgene Cat# 138715; http://n2t.net/addgene:138715; RRID:Addgene_138715 pCRISPaint-HaloTag-PuroR Addgene Cat# 80960; http://n2t.net/addgene:80960; RRID:Addgene_80960 Software and Algorithms OMERO OME Remote Objects software RRID:SCR_002629 Image Lab Software (Version 5.2.1) Bio-Rad RRID:SCR_014210 Biorender Software Science Suite Inc. RRID:SCR_018361 Prism 8 (Version 8.4.2) Graphpad Software RRID:SCR_002798 FIJI Open source RRID:SCR_002285 Xcalibur (Version 4.0) Thermo Scientific RRID:SCR_014593 MaxQuant (Version 1.6.10.43) Max-Planck-Institute of Biochemistry RRID:SCR_014485 Other CO2 Incubator C150 Binder Cat# 9040-0078 Sonicator, VibraCell- 72405 BioBlock scientific N/A KNF LABOPORT Mini Diaphragm Vacuum Pump N 811 in Pumps, Compressors Dominique Dutscher Cat# KNF_28002 Centrifuge Hettich Mikro 200 Grosseron N/A Mini-PROTEAN Tetra Vertical Electrophoresis Cell Bio-Rad Cat# 1658004 PowerPac HC High-Current Power Supply Bio-Rad Cat# 1645052 Trans-Blot Turbo Transfer System Bio-Rad Cat# 1704150 Slot Blot blotting manifold Hoefer Cat# PR648 Chemical Duty Pump, 220 V/50 Hz Merck Cat# WP6122050 Acclaim PepMap 100 C18 HPLC Columns ThermoFisher Scientific Cat# 164568 e4 Molecular Cell 83, 1640–1658.e1–e9, May 18, 2023

    Staining:

    Article Title: Design of Artificial Alcohol Oxidases: Alcohol Dehydrogenase–NADPH Oxidase Fusions for Continuous Oxidations
    Article Snippet: .. The samples were loaded onto a precast SDS‐PAGE gel (GenScript, USA), and the gels were run in a Mini‐PROTEAN Tetra Vertical Electrophoresis Cell (Bio‐Rad) with the current being applied by using a PowerPac HC High‐Current Power Supply (Bio‐Rad) set at 120 V. When the blue front of the loading dye reached the bottom of the gel, after about 70 min, the gel was removed from the chamber, and stained with Coomassie InstantBlue (Expedeon, US). .. An UV/Vis absorption spectrum from 200 to 700 nm (V‐330 Spectrophotometer, JASCO) was taken of each purified fusion protein diluted 1:10 in buffer (50 m m Tris ⋅ HCl, pH 8) in a quartz cuvette (Hellma Analytics, Germany).



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